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perineuronal nets pnns  (Bio-Rad)


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    Bio-Rad perineuronal nets pnns
    Perineuronal Nets Pnns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perineuronal+nets+pnns/Rat+anti+Mouse+CD68/bio_rxiv__64898__2026__03__25__714174-7-36-33
    Average 96 stars, based on 3261 article reviews
    perineuronal nets pnns - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice
    Article Snippet: .. Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs. .. Secondary antibodies included goat anti-rat Alexa Fluor 594 (Invitrogen, #A48264), goat anti-rabbit Alexa Fluor 635 (Invitrogen, #A31577), and goat anti-mouse Alexa Fluor 594 (Invitrogen, #A21125), as appropriate for each experiment.

    Labeling:

    Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice
    Article Snippet: .. Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs. .. Secondary antibodies included goat anti-rat Alexa Fluor 594 (Invitrogen, #A48264), goat anti-rabbit Alexa Fluor 635 (Invitrogen, #A31577), and goat anti-mouse Alexa Fluor 594 (Invitrogen, #A21125), as appropriate for each experiment.

    Marker:

    Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice
    Article Snippet: .. Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs. .. Secondary antibodies included goat anti-rat Alexa Fluor 594 (Invitrogen, #A48264), goat anti-rabbit Alexa Fluor 635 (Invitrogen, #A31577), and goat anti-mouse Alexa Fluor 594 (Invitrogen, #A21125), as appropriate for each experiment.



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    Image Search Results


    Methods use for immunofluorescence marker quantification and analysis

    Journal: Acta Neuropathologica Communications

    Article Title: Cognitively impaired aged Octodon degus recapitulate major neuropathological features of sporadic Alzheimer’s disease

    doi: 10.1186/s40478-022-01481-x

    Figure Lengend Snippet: Methods use for immunofluorescence marker quantification and analysis

    Article Snippet: Perineuronal nets (PNN) were immunostained using biotinylated Wisteria floribunda agglutinin (WFA; Vector Laboratories, cat# B-1355-2).

    Techniques: Immunofluorescence, Marker, Activity Assay

    Increased perineuronal nets signal coupled with an increase of PV-positive interneurons in the AD-like aged degu brain. Confocal microscopic views of degu coronal brain sections are aligned as indicated and show focused views of perineuronal nets (PNN) (green), parvalbumin (PV) interneurons (red), and neuronal nuclei (NeuN, blue) in entorhinal cortex (EC, a and d ), hippocampal CA2 and CA3 (Hippo, b and e ), and thalamic reticular nucleus (TRN, c and f ). g – l Quantification shows significantly increased counts of PNN(+)-neurons per mm 2 ( g ) and PV(+)-neurons per mm 2 ( h ) in EC ( p = 0.0022 and p 0.0260, respectively) in AD-like degu brains compared to Non-AD controls, along with similar trends for hippocampal CA3/CA2 ( p = 0.0931 for PNN; p = 0.3636 for PV) and TRN ( p = 0.3939 for PNN; p = 0.5887 for PV). Error bars represent SEM; * p < 0.05, ** p < 0.01, n = 6 degus in AD-like and Non-AD groups, n = 3 for male AD-like and female AD-like groups

    Journal: Acta Neuropathologica Communications

    Article Title: Cognitively impaired aged Octodon degus recapitulate major neuropathological features of sporadic Alzheimer’s disease

    doi: 10.1186/s40478-022-01481-x

    Figure Lengend Snippet: Increased perineuronal nets signal coupled with an increase of PV-positive interneurons in the AD-like aged degu brain. Confocal microscopic views of degu coronal brain sections are aligned as indicated and show focused views of perineuronal nets (PNN) (green), parvalbumin (PV) interneurons (red), and neuronal nuclei (NeuN, blue) in entorhinal cortex (EC, a and d ), hippocampal CA2 and CA3 (Hippo, b and e ), and thalamic reticular nucleus (TRN, c and f ). g – l Quantification shows significantly increased counts of PNN(+)-neurons per mm 2 ( g ) and PV(+)-neurons per mm 2 ( h ) in EC ( p = 0.0022 and p 0.0260, respectively) in AD-like degu brains compared to Non-AD controls, along with similar trends for hippocampal CA3/CA2 ( p = 0.0931 for PNN; p = 0.3636 for PV) and TRN ( p = 0.3939 for PNN; p = 0.5887 for PV). Error bars represent SEM; * p < 0.05, ** p < 0.01, n = 6 degus in AD-like and Non-AD groups, n = 3 for male AD-like and female AD-like groups

    Article Snippet: Perineuronal nets (PNN) were immunostained using biotinylated Wisteria floribunda agglutinin (WFA; Vector Laboratories, cat# B-1355-2).

    Techniques:

    Journal: Cell Reports

    Article Title: Transient developmental imbalance of cortical interneuron subtypes presages long-term changes in behavior

    doi: 10.1016/j.celrep.2021.109249

    Figure Lengend Snippet:

    Article Snippet: For detection of perineuronal nets Biotinylated Wisteria Floribunda Lectin (1:1000, Cat # B-1355, Vector labs) was applied on sections for 1 hour, followed by Streptavidin-647 (Cat #S32357, Life Technologies).

    Techniques: Recombinant, Plasmid Preparation, Imaging, Amplification, Software